Facultative Symbiont Infections Affect Aphid Reproduction

Jean-Christophe Simon (1) , Sébastien Boutin (1) , Tsutomu Tsuchida (2) , Ryuichi Koga (2) , Jean-François Le Gallic (1) , Adrien Frantz (3) , Yannick Outreman (1) , Takema Fukatsu (2)

1 BIO3P - Biologie des organismes et des populations appliquées à la protection des plantes
2 AIST - National Institute of Advanced Industrial Science and Technology
3 Laboratoire Ecologie et évolution

Some bacterial symbionts alter their hosts reproduction through various mechanisms that enhance their transmission in the host population. In addition to its obligatory symbiont Buchnera aphidicola, the pea aphid Acyrthosiphon pisum harbors several facultative symbionts influencing several aspects of host ecology. Aphids reproduce by cyclical parthenogenesis whereby clonal and sexual reproduction alternate within the annual life cycle. Many species, including the pea aphid, also show variation in their reproductive mode at the population level, with some lineages reproducing by cyclical parthenogenesis and others by permanent parthenogenesis. While the role of facultative symbionts has been well studied during the parthenogenetic phase of their aphid hosts, very little is known on their possible influence during the sexual phase. Here we investigated whether facultative symbionts modulate the capacity to produce sexual forms in various genetic backgrounds of the pea aphid with controlled symbiont composition and also in different aphid genotypes from natural populations with previously characterized infection status and reproductive mode. We found that most facultative symbionts exhibited detrimental effects on their hosts fitness under sex-inducing conditions in comparison with the reference lines. We also showed that the loss of sexual phase in permanently parthenogenetic lineages of A. pisum was not explained by facultative symbionts. Finally, we demonstrated that Spiroplasma infection annihilated the production of males in the host progeny by inducing a male-killing phenotype, an unexpected result for organisms such as aphids that reproduce primarily through clonal reproduction.

Mots clés: acyrthosiphon-pisum harris; male-killing bacteria; life-history traits; pea aphid; horizontal transfer; host-populations; ecological; specialization; endosymbiotic bacteria; genetic-variation; myzus-persicae

Domaines: Biologie végétale

Jean-Christophe Simon, Sébastien Boutin, Tsutomu Tsuchida, Ryuichi Koga, Jean-François Le Gallic, et al.. Facultative Symbiont Infections Affect Aphid Reproduction. PLoS ONE, 2011, 6 (7), non paginé. ⟨hal-00729321⟩