Analysis of helicase-RNA interactions using nucleotide analog interference mapping.

Abstract : Nucleotide analog interference mapping (NAIM) is a combinatorial approach that probes individual atoms and functional groups in an RNA molecule and identifies those that are important for a specific biochemical function. Here, we show how NAIM can be adapted to reveal functionally important atoms and groups on RNA substrates of helicases. We explain how NAIM can be used to investigate translocation and unwinding mechanisms of helicases and discuss the advantages and limitations of this powerful chemogenetic approach.
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Submitted on : Wednesday, August 29, 2012 - 2:37:31 PM
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Annie Schwartz, Makhlouf Rabhi, Emmanuel Margeat, Marc Boudvillain. Analysis of helicase-RNA interactions using nucleotide analog interference mapping.. Methods in Enzymology, Elsevier, 2012, 511, pp.149-69. ⟨10.1016/B978-0-12-396546-2.00007-3⟩. ⟨hal-00726256⟩

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