Investigation of RNA-Ligand Interactions by (19) F NMR Spectroscopy Using Fluorinated Probes.

Thomas Lombès , Roba Moumné (1, 2) , Valéry Larue (3) , Elise Prost (4) , Marjorie Catala , Thomas Lecourt (4) , Frédéric Dardel (3) , Laurent Micouin (4) , Carine Tisné (3)

1 SSFMB - Synthèse, Structure et Fonction de Molécules Bioactives
2 UPMC - Université Pierre et Marie Curie - Paris 6
3 LCRB - UMR 8015 - Laboratoire de cristallographie et RMN biologiques
4 SSMIP - Synthèse et structure de molécules d'interet pharmacologique

Spy swap: The interaction between an unlabeled RNA and unlabeled ligands (red hexagon) can be monitored by (19) F NMR spectroscopy using small fluorinated diamines (green star) as spy reporters. This technique also enables the visualization of the conformational capture of a riboswitch by its ligand.

Domaines: Chimie organique

Thomas Lombès, Roba Moumné, Valéry Larue, Elise Prost, Marjorie Catala, et al.. Investigation of RNA-Ligand Interactions by (19) F NMR Spectroscopy Using Fluorinated Probes.. Angewandte Chemie International Edition, 2012, 51 (38), pp.9530-9534. ⟨10.1002/anie.201204083⟩. ⟨hal-00724894⟩