A fraction of neurofibromin interacts with PML bodies in the nucleus of the CCF astrocytoma cell line.

Fabienne Godin (1) , Sandrine Villette (1) , Béatrice Vallée (1) , Michel Doudeau (1) , Séverine Morisset-Lopez (1) , Maryvonne Ardourel , Tobias Hevor (2) , Chantal Pichon (1) , Hélène Bénédetti (1)

1 CBM - Centre de biophysique moléculaire
2 Laboratoire de Neurobiologie

Neurofibromatosis type 1 is a common genetic disease that causes nervous system tumors, and cognitive deficits. It is due to mutations within the NF1 gene, which encodes the Nf1 protein. Nf1 has been shown to be involved in the regulation of Ras, cAMP and actin cytoskeleton dynamics. In this study, using immunofluorescence experiments, we have shown a partial nuclear localization of Nf1 in the astrocytoma cell line: CCF and we have demonstrated that Nf1 partially colocalizes with PML (promyelocytic leukemia) nuclear bodies. A direct interaction between Nf1 and the multiprotein complex has further been demonstrated using "in situ" proximity ligation assay (PLA).

Mots clés: Nf1; Nucleus; PML bodies; Immunolocalization; PLA technique; NF1 TUMOR-SUPPRESSOR; GAP-RELATED DOMAIN; GENE-PRODUCT; ADENYLYL-CYCLASE; TYPE-1; PATHWAY; PROTEIN; HETEROZYGOSITY; INACTIVATION; DIFFERENTIATION

Domaines: Biochimie, Biologie Moléculaire

Fabienne Godin, Sandrine Villette, Béatrice Vallée, Michel Doudeau, Séverine Morisset-Lopez, et al.. A fraction of neurofibromin interacts with PML bodies in the nucleus of the CCF astrocytoma cell line.. Biochemical and Biophysical Research Communications, 2012, 418 (4), pp.689-94. ⟨10.1016/j.bbrc.2012.01.079⟩. ⟨hal-00721861⟩