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Thidiazuron-induced shoot organogenesis from mature leaf explants of scented Pelargonium capitatum cultivars

Abstract : Shoot organogenesis from mature leaf tissues of two scented Pelargonium capitatum cultivars, 'Attar of Roses' and 'Atomic Snowflake', grown in the greenhouse, were optimized in the presence of thidiazuron (TDZ). The protocol involved preculture of leaf sections on basal Murashige and Skoog (MS) medium supplemented with 10 lM TDZ, 4.4 lM of 6-benzyladenine (BA) and 5.4 lM a-naphtaleneacetic acid (NAA) for a period of 2 weeks and followed by subculture of explants to a fresh medium containing 4.4 lM BA and 5.4 lM NAA. Frequency of regeneration reached approximately 93% for both cultivars, with the induction of more than 100 shoots per explant. Regenerated plantlets were rooted on half-strength MS medium supplemented with 4.4 mM sucrose and 8.6 lM of Indole-3-acetic acid (IAA). All regenerated shoots from both cultivars developed roots when transferred to organic soil mix, acclimatized, and successfully transferred to greenhouse conditions. When regenerated shoots were transferred to hydroponic conditions, frequency of survival was 76.2 and 61.9% for 'Attar of Roses' and 'Atomic Snowflake', respectively.
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Submitted on : Wednesday, July 11, 2012 - 5:40:59 PM
Last modification on : Tuesday, December 14, 2021 - 3:38:06 PM
Long-term archiving on: : Friday, October 12, 2012 - 3:10:16 AM

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  • HAL Id : hal-00716999, version 1
  • OATAO : 5820

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Muhammad Arshad, Jérôme Silvestre, Georges Merlina, Camille Dumat, Eric Pinelli, et al.. Thidiazuron-induced shoot organogenesis from mature leaf explants of scented Pelargonium capitatum cultivars. Plant Cell, Tissue and Organ Culture, Springer Verlag, 2012, vol. 108, pp. 315-322. ⟨hal-00716999⟩

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