Optimized conditions for the synthesis of vanillic acid under hypersaline conditions by Halomonas elongata DSM 2581(T) resting cells

Abstract : During growth on ferulic acid, Halomonas elongata DSM 2581(T) was capable of promoting the formation of a significant amount of vanillic acid. The products were confirmed by high-performance liquid chromatography and gas chromatography mass-spectrometry analyses. To enhance the formation of vanillic acid and prevent its degradation, a resting-cell method using Halomonas elongata was developed. The growth state of the culture utilized for biomass production, the concentration of the biomass, the amount of ferulic acid that was treated and the reutilization of the biomass were optimized. The optimal yield of vanillic acid (82%) was obtained after a 10-h reaction using 10 mM ferulic acid and 5 g/l of cell pregrown on ferulic acid and harvested at the end of the exponential phase.
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WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2008, 24, pp.675-680. 〈10.1007/s11274-007-9523-3〉
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Soumis le : lundi 2 juillet 2012 - 12:19:44
Dernière modification le : mercredi 4 juillet 2018 - 11:08:02

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Slim Abdelkafi, Marc Labat, Zouhaier Ben Ali Gam, Jean Lorquin, Laurie Casalot, et al.. Optimized conditions for the synthesis of vanillic acid under hypersaline conditions by Halomonas elongata DSM 2581(T) resting cells. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2008, 24, pp.675-680. 〈10.1007/s11274-007-9523-3〉. 〈hal-00713655〉

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