Skip to Main content Skip to Navigation
Journal articles

M19 modulates skeletal muscle differentiation and insulin secretion in pancreatic β-cells through modulation of respiratory chain activity.

Abstract : Mitochondrial dysfunction due to nuclear or mitochondrial DNA alterations contributes to multiple diseases such as metabolic myopathies, neurodegenerative disorders, diabetes and cancer. Nevertheless, to date, only half of the estimated 1,500 mitochondrial proteins has been identified, and the function of most of these proteins remains to be determined. Here, we characterize the function of M19, a novel mitochondrial nucleoid protein, in muscle and pancreatic β-cells. We have identified a 13-long amino acid sequence located at the N-terminus of M19 that targets the protein to mitochondria. Furthermore, using RNA interference and over-expression strategies, we demonstrate that M19 modulates mitochondrial oxygen consumption and ATP production, and could therefore regulate the respiratory chain activity. In an effort to determine whether M19 could play a role in the regulation of various cell activities, we show that this nucleoid protein, probably through its modulation of mitochondrial ATP production, acts on late muscle differentiation in myogenic C2C12 cells, and plays a permissive role on insulin secretion under basal glucose conditions in INS-1 pancreatic β-cells. Our results are therefore establishing a functional link between a mitochondrial nucleoid protein and the modulation of respiratory chain activities leading to the regulation of major cellular processes such as myogenesis and insulin secretion.
Complete list of metadatas

Cited literature [24 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-00706794
Contributor : Anne Dominique Lajoix <>
Submitted on : Friday, May 29, 2020 - 11:13:45 AM
Last modification on : Friday, September 4, 2020 - 2:46:03 PM

File

2012_Cambier_Plos One_1.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Linda Cambier, Patrice Rassam, Béatrice Chabi, Karima Mezghenna, René Gross, et al.. M19 modulates skeletal muscle differentiation and insulin secretion in pancreatic β-cells through modulation of respiratory chain activity.. Clinical Neurology and Neurosurgery, Elsevier, 2012, 7 (2), pp.e31815. ⟨10.1371/journal.pone.0031815⟩. ⟨hal-00706794⟩

Share

Metrics

Record views

190

Files downloads

25