Label free and amplified detection of cancer marker EBNA-1 by DNA probe based biosensors

Abstract : Epstein-Barr virus (EBV) is a human herpes virus that has been associated with several malignancies as Burkitt's lymphoma, nasopharyngeal carcinoma and Hodgkin's disease. All EBV associated malignancies showed a distinct viral gene expression pattern, while Epstein-Barr nuclear antigen 1 (EBNA-1) is constitutively expressed in all such disorders. Here, the development of a biosensor to detect EBNA-1 protein is reported, which was based on a nucleic acid bioreceptor and a quartz crystal microbalance with a dissipation monitoring (QCM-D) transducer. The DNA probe for EBNA-1 detection was designed and synthesized to mimic its palindromic target sites in the EBV genome. This DNA probe was immobilized on the Au-surface of a QCM-D electrode, followed by the blocking of the accessible Au-surface with 6-mercapto-1-hexanol (6-MHO). The system showed a limit of detection of 50 ng/mL in direct detection of EBNA-1, however, the sensitivity was improved by 2 orders of magnitude (0.5 ng/mL) when an amplification cascade, employing antibodies labeled with alkaline phosphatase (AP), was applied to the system.
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https://hal.archives-ouvertes.fr/hal-00704075
Contributor : Géraldine Rohman <>
Submitted on : Monday, June 4, 2012 - 5:26:22 PM
Last modification on : Saturday, February 15, 2020 - 2:05:27 AM

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G. Garai-Ibabe, R. Grinyte, E. I. Golub, A. Canaan, Marc Lamy de la Chapelle, et al.. Label free and amplified detection of cancer marker EBNA-1 by DNA probe based biosensors. Biosensors & bioelectronics, 2011, 30 (1), pp.272-275. ⟨10.1016/j.bios.2011.09.025⟩. ⟨hal-00704075⟩

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