An automated approach for single-cell tracking in epifluorescence microscopy applied to E. coli growth analysis on microfluidics biochips

Abstract : With the accumulation of knowledge for the intimate molecular mechanisms governing the processes inside the living cells in the later years, the ability to characterize the performance of elementary genetic circuits and parts at the single-cell level is becoming of crucial importance. Biological science is arriving to the point where it can develop hypothesis for the action of each molecule participating in the biochemical reactions and need proper techniques to test those hypothesis. Microfluidics is emerging as the technology that combined with high-magnification microscopy will allow for the long-term single-cell level observation of bacterial physiology. In this study we design, build and characterize the gene dynamics of genetic circuits as one of the basic parts governing programmed cell behavior. We use E. coli as model organism and grow it in microfluidics chips, which we observe with epifluorescence microscopy. One of the most invaluable segments of this technology is the consequent image processing, since it allows for the automated analysis of vast amount of single-cell observation and the fast and easy derivation of conclusions based on that data. Specifically, we are interested in promoter activity as function of time. We expect it to be oscillatory and for that we use GFP (green fluorescent protein) as a reporter in our genetic circuits. In this paper, an automated framework for single-cell tracking in phase-contrast microscopy is developed, combining 2D segmentation of cell time frames and graph-based reconstruction of their spatiotemporal evolution with fast tracking of the associated fluorescence signal. The results obtained on the investigated biological database are presented and discussed.
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Communication dans un congrès
Biomedical Applications in Molecular, Structural, and Functional Imaging, Feb 2012, San Diego, United States. 8317, pp.83170Z:1-11, 2012, <10.1117/12.911371>
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https://hal.archives-ouvertes.fr/hal-00697395
Contributeur : Catalin Fetita <>
Soumis le : mardi 15 mai 2012 - 11:41:59
Dernière modification le : jeudi 9 février 2017 - 15:22:54

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Catalin Fetita, Boris Kirov, Alfonso Jaramillo. An automated approach for single-cell tracking in epifluorescence microscopy applied to E. coli growth analysis on microfluidics biochips. Biomedical Applications in Molecular, Structural, and Functional Imaging, Feb 2012, San Diego, United States. 8317, pp.83170Z:1-11, 2012, <10.1117/12.911371>. <hal-00697395>

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