Impact of immobilization support on colorimetric microarrays performances.

Abstract : We report here a comparison of support materials for colorimetric hybridization assays on microarrays. Four surfaces with various chemistries and architectures (roughness and porosity) were evaluated: (i) bare and (ii) activated polystyrene surfaces classically used for ELISA; (iii) a double-sided adhesive support; and (iv) a porous nitrocellulose/cellulose acetate membrane. Each substrate was functionalized with a microarray of probes and subjected to an enzymatic colorimetric DNA hybridization test. Tests were carried out in a 96-well assembly suitable for automated high-throughput analysis. Colorimetry results, microscopy observations and a chemiluminescence study showed that the test efficiency not only depends on the surface probe density but that the capacity of the material to retain the colored enzymatic product is also a critical parameter. All parameters being considered, the adhesive coated surface proposes the best surface properties for efficient colorimetric microarrays.
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Biosensors and Bioelectronics, Elsevier, 2012, 35 (1), pp.94-100. 〈10.1016/j.bios.2012.02.025〉
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https://hal.archives-ouvertes.fr/hal-00695728
Contributeur : Frédérique Depierre <>
Soumis le : mercredi 9 mai 2012 - 16:50:00
Dernière modification le : mercredi 12 décembre 2018 - 15:21:27

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Gaelle C Le Goff, Benjamin P Corgier, Céline A Mandon, Gabriel De Crozals, Carole Chaix, et al.. Impact of immobilization support on colorimetric microarrays performances.. Biosensors and Bioelectronics, Elsevier, 2012, 35 (1), pp.94-100. 〈10.1016/j.bios.2012.02.025〉. 〈hal-00695728〉

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