Impact of immobilization support on colorimetric microarrays performances.

Abstract : We report here a comparison of support materials for colorimetric hybridization assays on microarrays. Four surfaces with various chemistries and architectures (roughness and porosity) were evaluated: (i) bare and (ii) activated polystyrene surfaces classically used for ELISA; (iii) a double-sided adhesive support; and (iv) a porous nitrocellulose/cellulose acetate membrane. Each substrate was functionalized with a microarray of probes and subjected to an enzymatic colorimetric DNA hybridization test. Tests were carried out in a 96-well assembly suitable for automated high-throughput analysis. Colorimetry results, microscopy observations and a chemiluminescence study showed that the test efficiency not only depends on the surface probe density but that the capacity of the material to retain the colored enzymatic product is also a critical parameter. All parameters being considered, the adhesive coated surface proposes the best surface properties for efficient colorimetric microarrays.
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Journal articles
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https://hal.archives-ouvertes.fr/hal-00695728
Contributor : Frédérique Depierre <>
Submitted on : Wednesday, May 9, 2012 - 4:50:00 PM
Last modification on : Wednesday, December 12, 2018 - 3:21:27 PM

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Gaelle C Le Goff, Benjamin P Corgier, Céline A Mandon, Gabriel de Crozals, Carole Chaix, et al.. Impact of immobilization support on colorimetric microarrays performances.. Biosensors and Bioelectronics, Elsevier, 2012, 35 (1), pp.94-100. ⟨10.1016/j.bios.2012.02.025⟩. ⟨hal-00695728⟩

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