Skip to Main content Skip to Navigation
Conference papers

Blind deconvolution of 3D data in wide field fluorescence microscopy

Abstract : In this paper we propose a blind deconvolution algorithm for wide field fluorescence microscopy. The 3D PSF is modeled after a parametrized pupil function. The PSF parameters are estimated jointly with the object in a maximum a posteriori framework. We illustrate the performances of our algorithm on experimental data and show significant resolution improvement notably along the depth. Quantitative measurements on images of calibration beads demonstrate the benefits of blind deconvolution both in terms of contrast and resolution compared to non-blind deconvolution using a theoretical PSF.
Complete list of metadatas

Cited literature [15 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-00691249
Contributor : Ferréol Soulez <>
Submitted on : Wednesday, April 25, 2012 - 5:46:15 PM
Last modification on : Monday, January 13, 2020 - 5:46:04 PM
Document(s) archivé(s) le : Monday, November 26, 2012 - 3:46:20 PM

File

PapierISBI.pdf
Files produced by the author(s)

Identifiers

  • HAL Id : hal-00691249, version 1

Citation

Ferréol Soulez, Loïc Denis, Yves Tourneur, Éric Thiébaut. Blind deconvolution of 3D data in wide field fluorescence microscopy. International Symposium on Biomedical Imaging, May 2012, Barcelone, Spain. pp.CDROM. ⟨hal-00691249⟩

Share

Metrics

Record views

567

Files downloads

1673