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Blind deconvolution of 3D data in wide field fluorescence microscopy

Abstract : In this paper we propose a blind deconvolution algorithm for wide field fluorescence microscopy. The 3D PSF is modeled after a parametrized pupil function. The PSF parameters are estimated jointly with the object in a maximum a posteriori framework. We illustrate the performances of our algorithm on experimental data and show significant resolution improvement notably along the depth. Quantitative measurements on images of calibration beads demonstrate the benefits of blind deconvolution both in terms of contrast and resolution compared to non-blind deconvolution using a theoretical PSF.
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Contributor : Ferréol Soulez <>
Submitted on : Wednesday, April 25, 2012 - 5:46:15 PM
Last modification on : Friday, June 11, 2021 - 7:44:02 PM
Long-term archiving on: : Monday, November 26, 2012 - 3:46:20 PM


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  • HAL Id : hal-00691249, version 1


Ferréol Soulez, Loïc Denis, Yves Tourneur, Éric Thiébaut. Blind deconvolution of 3D data in wide field fluorescence microscopy. International Symposium on Biomedical Imaging, May 2012, Barcelone, Spain. pp.CDROM. ⟨hal-00691249⟩



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