Methodology for reconstructing early zebrafish development from in vivo multiphoton microscopy.

Abstract : Investigating cell dynamics during early zebrafish embryogenesis requires specific image acquisition and analysis strategies. Multiharmonic microscopy, i.e., second- and third-harmonic generations, allows imaging cell divisions and cell membranes in unstained zebrafish embryos from 1- to 1000-cell stage. This paper presents the design and implementation of a dedicated image processing pipeline (tracking and segmentation) for the reconstruction of cell dynamics during these developmental stages. This methodology allows the reconstruction of the cell lineage tree including division timings, spatial coordinates, and cell shape until the 1000-cell stage with minute temporal accuracy and micrometer spatial resolution. Data analysis of the digital embryos provides an extensive quantitative description of early zebrafish embryogenesis.
Document type :
Journal articles
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-00683640
Contributor : Alain Perignon <>
Submitted on : Thursday, March 29, 2012 - 2:26:46 PM
Last modification on : Wednesday, May 15, 2019 - 4:06:18 AM

Links full text

Identifiers

Citation

Miguel A. Luengo-Oroz, José L. Rubio-Guivernau, Emmanuel Faure, Thierry Savy, Louise Duloquin, et al.. Methodology for reconstructing early zebrafish development from in vivo multiphoton microscopy.. IEEE Transactions on Image Processing, Institute of Electrical and Electronics Engineers, 2012, 21 (4), pp.2335-40. ⟨10.1109/TIP.2011.2177911⟩. ⟨hal-00683640⟩

Share

Metrics

Record views

1211