Multicenter collaborative study for standardization of Candida albicans genotyping using a polymorphic microsatellite marker.

Dea Garcia-Hermoso (1) , Donna M Maccallum , Timothy J Lott , Paula Sampaio , Maria-José Buitrago Serna , Fréderic Grenouillet (2) , Corné H W Klaassen , Stéphane Bretagne (1, 3, 4)

1 CNRMA - Centre National de Référence Mycologie et Antifongiques-Mycologie Moléculaire
2 LCE - Laboratoire Chrono-environnement (UMR 6249)
3 Hôpital Henri Mondor
4 BIPAR - Biologie moléculaire et immunologie parasitaires et fongiques

Microsatellite-based genotyping for Candida albicans can give discrepant results between laboratories when expressed in fragment sizes, because their determination depends on electrophoretic conditions. The interlaboratory reproducibility was assessed in six laboratories provided with an allelic ladder. Despite variations in size determinations, alleles were correctly assigned, making data transportable between laboratories.

Mots clés: Alleles; Candida albicans; Genotype; Laboratories; Microsatellite Repeats; Polymorphism; Genetic; Reference Standards; Reproducibility of Results

Mots clés: Bacterial Typing Techniques; Molecular Diagnostic Techniques

Domaines: Mycologie

Dea Garcia-Hermoso, Donna M Maccallum, Timothy J Lott, Paula Sampaio, Maria-José Buitrago Serna, et al.. Multicenter collaborative study for standardization of Candida albicans genotyping using a polymorphic microsatellite marker.. Journal of Clinical Microbiology, 2010, 48 (7), pp.2578-81. ⟨10.1128/JCM.00040-10⟩. ⟨hal-00682520⟩