Dynamic aberration correction for multiharmonic microscopy.

Abstract : We demonstrate image-based aberration correction in a third-harmonic generation (THG) microscope. We describe a robust, mostly sample-independent correction scheme relying on prior measurement of the influence of aberration modes produced by a deformable mirror on the quality of THG images. We find that using image sharpness as an image quality metric, correction of N aberration modes is achieved using 2(2N+1) measurements in a variety of samples. We also report aberration correction in combined multiharmonic and two-photon excited fluorescence experiments. Finally, we demonstrate time-dependent adaptive THG imaging in developing embryonic tissue.
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Nicolas Olivier, Delphine Débarre, Emmanuel Beaurepaire. Dynamic aberration correction for multiharmonic microscopy.. Optics Letters, Optical Society of America, 2009, 34 (20), pp.3145-7. ⟨hal-00681948⟩

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