The role of distribution and orientation of collagen fibers in tissue development: study by means of double imaging by two-photon excited fluorescence and second harmonic generation microscopy

Olena P. Boryskina (1) , Yann Le Grand (2, 3) , Christophe Odin (2) , Vincent Fleury (4, 5)

1 NASU / НАН України - National Academy of Sciences of Ukraine = Національна академія наук України = Académie nationale des sciences d'Ukraine
2 IPR - Institut de Physique de Rennes
3 LSOL - Laboratoire de Spectrométrie et Optique Laser
4 LMFA - Laboratoire de Mecanique des Fluides et d'Acoustique
5 MSC - Matière et Systèmes Complexes

The TPEF and SHG imaging was used to study the distribution and orientation of collagen fibers in immature skin appendages on ex vivo chicken embryo skin samples in connection to mechanical properties of developing tissues. Our study has demonstrated that the orientation of collagen fibers in the feather buds may promote growth of feathers by creating the gradient of stiffness and thus triggering the pressure sensitive growth factors.

Domaines: Biochimie, Biologie Moléculaire

Olena P. Boryskina, Yann Le Grand, Christophe Odin, Vincent Fleury. The role of distribution and orientation of collagen fibers in tissue development: study by means of double imaging by two-photon excited fluorescence and second harmonic generation microscopy. 38th European Microwave Conference 2008, Oct 2008, Amsterdam, Netherlands. pp.255-259. ⟨hal-00672507⟩