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Article Dans Une Revue Journal of Biophysical Journal Année : 2011

Kinetic and binding equilibrium studies of dihydroflavonol 4-reductase from Vitis vinifera and its unusually strong substrate inhibition

Résumé

Dihydroflavonol 4-reductase (DFR), a member of the short-chain dehydrogenase family, catalyzes the last common step in the biosynthesis of flavan-3-ols and condensed tannins. Initial rates of DFR were measured by monitoring the 340- nm absorbance decrease resulting from the joint consumption of dihydroquercetin (DHQ) and NADPH, as a function of pH, temperature and ionic strength. At pH 6.5 and 30°C, substrate inhibition was observed above 30 μM DHQ. At lower/non-inhibitory DHQ concentrations, NADP+ behaves as a competitive inhibitor with respect to NADPH and as a mixed inhibitor with respect to DHQ, which supports a sequential ordered mechanism, with NADPH binding first and NADP+ released last. Binding-equilibrium data obtained by means of the chromatographic method of Hummel and Dreyer at pH 7.5 and by isothermal calorimetric titration at pH 6.5 led to the conclusion that ligands of the apoenzyme included NADPH, NADP+ and DHQ. The mecha-nism which best accounts for substrate inhibi-tion at pH 6.5 in the absence of product involves the formation of a binary non-productive E.DHQ complex. Thus, a productive ternary complex cannot be formed when DHQ binds first. This mechanism of inhibition may prevent the ac-cumulation of unstable leucoanthocyanidins within cells.

Dates et versions

hal-00663424 , version 1 (27-01-2012)

Identifiants

Citer

Nadia Trabelsi, Béatrice Langlois d'Estaintot, Gilles Sigaud, Bernard Gallois, Jean Chaudière. Kinetic and binding equilibrium studies of dihydroflavonol 4-reductase from Vitis vinifera and its unusually strong substrate inhibition. Journal of Biophysical Journal, 2011, 2 (3), pp. 332-344. ⟨10.4236/jbpc.2011.23038⟩. ⟨hal-00663424⟩

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