Skip to Main content Skip to Navigation
Journal articles

Adaptive Optics for Fluorescence Correlation Spectroscopy

Charles Edouard Leroux 1 Irène Wang 1 Jacques Derouard 1 Antoine Delon 1
1 MOTIV
LIPhy [2011-2015] - Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères] [2011-2015]
Abstract : Fluorescence Correlation Spectroscopy (FCS) yields measurement parameters (number of molecules, diffusion time) that characterize the concentration and kinetics of fluorescent molecules within a supposedly known observation volume. Absolute derivation of concentrations and diffusion constants therefore requires preliminary calibrations of the confocal Point Spread Function with phantom solutions under perfectly controlled environmental conditions. In this paper, we quantify the influence of optical aberrations on single photon FCS and demonstrate a simple Adaptive Optics system for aberration correction. Optical aberrations are gradually introduced by focussing the excitation laser beam at increasing depths in fluorescent solutions with various refractive indices, which leads to drastic depth-dependent bias in the estimated FCS parameters. Aberration correction with a Deformable Mirror stabilizes these parameters within a range of several tens of μm into the solution. We also demonstrate, both theoretically and experimentally, that the molecular brightness scales as the Strehl ratio squared.
Document type :
Journal articles
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-00632850
Contributor : Antoine Delon <>
Submitted on : Monday, October 17, 2011 - 3:31:47 PM
Last modification on : Tuesday, October 6, 2020 - 4:30:48 PM
Long-term archiving on: : Wednesday, January 18, 2012 - 2:20:28 AM

Files

aofcs.pdf
Files produced by the author(s)

Identifiers

Collections

UGA | CNRS | LIPHY

Citation

Charles Edouard Leroux, Irène Wang, Jacques Derouard, Antoine Delon. Adaptive Optics for Fluorescence Correlation Spectroscopy. Optics Express, Optical Society of America - OSA Publishing, 2011, 19 (27), pp.26839. ⟨10.1364/OE.19.026839⟩. ⟨hal-00632850⟩

Share

Metrics

Record views

393

Files downloads

460