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Effect of various additives and polymers on lysozyme release from PLGA microspheres prepared by an s/o/w emulsion technique

Abstract : Incomplete protein release from PLGA-based microspheres due to protein interactions with the polymer is one of the main issues in the development of PLGA protein-loaded microspheres. In this study, a two-dimensional adsorption model was designed to rapidly assess the anti-adsorption effect of formulation components (additives, additives blended with the polymer or modified polymers). Lysozyme was chosen as a model protein because of its strong, non-specific adsorption on the PLGA surface. This study showed that PEGs, poloxamer 188 and BSA totally inhibited protein adsorption onto the PLGA37.5/25 layer. Similarly, it was emphasised that more hydrophilic polymers were less prone to protein adsorption. The correlation between this model and the in vitro release profile was made by microencapsulating lysozyme with a low loading in the presence of these excipients by a non-denaturing s/o/w encapsulation technique. The precipitation of lysozyme with the amphiphilic poloxamer 188 prior to encapsulation exhibited continuous release of active lysozyme over 3 weeks without any burst effect. To promote lysozyme release in the latter stage of release, a PLGA-PEG-PLGA tribloc copolymer was used; lysozyme was continuously released over 45 days in a biologically active form.
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https://hal.archives-ouvertes.fr/hal-00626154
Contributor : Brigitte Carles Connect in order to contact the contributor
Submitted on : Friday, September 23, 2011 - 4:05:32 PM
Last modification on : Wednesday, October 20, 2021 - 3:19:04 AM

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A. Paillard-Giteau, V.T. Tran, O. Thomas, X. Garric, J. Coudane, et al.. Effect of various additives and polymers on lysozyme release from PLGA microspheres prepared by an s/o/w emulsion technique. European Journal of Pharmaceutics and Biopharmaceutics, Elsevier, 2010, 75, pp.128-136. ⟨10.1016/j.ejpb.2010.03.005⟩. ⟨hal-00626154⟩

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