Deciphering fluorescence signals by quantifying separately the excitation intensity from the number of emitters

Abstract : Conventional fluorescence detection is sensitive to an intricate product of the number of fluorescent emitters times the local excitation intensity. Here, we describe a method to locally quantify the excitation intensity and the number of emitters separately, enabling a clear distinction between the phenomena responsible for a given fluorescence signal. Our technique is based on harmonic excitation modulation and higher-order fluorescence demodulation. It is compatible with a wide range of observations, opening new characterization routes for fluorescence detection or imaging on complex systems, such as plasmonic substrates or scattering media.
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Journal articles
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https://hal.archives-ouvertes.fr/hal-00616382
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Submitted on : Monday, August 22, 2011 - 1:19:06 PM
Last modification on : Friday, March 22, 2019 - 1:39:28 AM

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R. Hostein, H. Aouani, H. Rigneault, Jérôme Wenger. Deciphering fluorescence signals by quantifying separately the excitation intensity from the number of emitters. Optics Letters, Optical Society of America, 2011, 36 (17), pp.3317-3319. ⟨10.1364/OL.36.003317⟩. ⟨hal-00616382⟩

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