EVALUATION OF TYPE-SPECIFIC HPV PERSISTENCE AND HIGH-RISK HPV VIRAL LOAD QUANTITATION IN HPV POSITIVE WOMEN UNDER 30 WITH NORMAL CERVICAL CYTOLOGY - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Medical Virology Année : 2011

EVALUATION OF TYPE-SPECIFIC HPV PERSISTENCE AND HIGH-RISK HPV VIRAL LOAD QUANTITATION IN HPV POSITIVE WOMEN UNDER 30 WITH NORMAL CERVICAL CYTOLOGY

Noel Bolger
  • Fonction : Auteur
  • PersonId : 907720
Mireille Henry
  • Fonction : Auteur
  • PersonId : 886628
Julien Mancini
Leon Boubli
  • Fonction : Auteur
  • PersonId : 907721
Daniel Olive
  • Fonction : Auteur
  • PersonId : 849345
Sinead Cleary
  • Fonction : Auteur
  • PersonId : 907722
Walter Prendiville
  • Fonction : Auteur
  • PersonId : 907723
Catherine Tamalet
  • Fonction : Auteur
  • PersonId : 907724

Résumé

The persistence of high-risk HPV (HR-HPV) infection is necessary for the development of cervical intraepithelial neoplasia. The aim of this study was to evaluate if HR-HPV typing and HPV16, 18, 31 and 33 quantitation are predictive for type-specific infection persistence and/or the development of CIN in women under 30 with normal cervical cytology. Young women (under 30) attending a family planning clinic who were HPV positive with normal cervical cytology were included. HPV genotyping was assessed by MY09/ MY11 PCR, sequencing, phylogenetic analysis, and cloning where necessary. HR-HPV viral load was quantified using duplex real-time PCR. Study patients were offered for a second smear and HR-HPV detection and quantitation after twelve months. HR-HPV was identified in 43 (21.9%) of the 199 included women. Of these, 39 patients had a second cervical sample taken within a mean interval of 11.7 months (8.8-18.3 months). The mean HR-HPV 16, 18, 31 and 33 initial viral load was 1.9x106 copies per millions cells. The level of viral load did not reveal any significant association with type-specific HR-HPV persistence or the subsequent development of cervical intraepithelial neoplasia. Only HPV16 infection was significantly more likely to persist (91.7% vs. 33.1%, p=0.001) and to develop CIN (33.3% vs. 3.7%, p=0.025). In women under 30 with normal cytology, HR-HPV viral load is common and is not predictive of HPV persistence or the development of cervical intraepithelial neoplasia. HPV16 positive women are significantly more likely to have persistent infection and to develop cervical intraepithelial neoplasia.

Mots clés

Fichier principal
Vignette du fichier
PEER_stage2_10.1002%2Fjmv.22022.pdf (337.52 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-00614696 , version 1 (15-08-2011)

Identifiants

Citer

Xavier Carcopino, Noel Bolger, Mireille Henry, Julien Mancini, Leon Boubli, et al.. EVALUATION OF TYPE-SPECIFIC HPV PERSISTENCE AND HIGH-RISK HPV VIRAL LOAD QUANTITATION IN HPV POSITIVE WOMEN UNDER 30 WITH NORMAL CERVICAL CYTOLOGY. Journal of Medical Virology, 2011, 83 (4), pp.637. ⟨10.1002/jmv.22022⟩. ⟨hal-00614696⟩

Collections

PEER
69 Consultations
250 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More