Parvovirus 4 in French in-patients: a study of haemodialysis and lung transplant cohorts

Mhammed Touinssi (1, 2) , Martine Reynaud-Gaubert (3) , Carine Gomez (3) , Pascal Thomas (3) , Bertrand Dussol (4) , Yvon Berland (4) , Agnes Basire (1) , Christophe Picard (1) , Jean François Cantaloube (2, 1) , Philippe de Micco (1, 2) , Philippe Biagini (1, 2)

1 EFS - Alpes-Méditerranée - Etablissement Français du Sang - Alpes-Méditerranée
2 UAABC - Anthropologie Bio-Culturelle
3 Hôpital Nord [CHU - APHM]
4 Chirurgie urologique et transplantation rénale [Hôpital de la Conception - APHM]

The epidemiology and the clinical implication of human parvovirus 4 (PARV4) in human populations is still under evaluation. The distribution of PARV4 DNA was determined in cohorts of French haemodialysis and lung transplant patients. Plasma samples (n=289) were tested for PARV4 by real-time PCR assay (ORF2), and amplification products selected at random were sequenced. Analysis of available serological and biological markers was also undertaken. Fifty-seven samples out of 185 (30.8%) were positive for PARV4 DNA in the cohort of haemodialysis patients. A higher prevalence of the virus was identified in individuals with markers of HBV infection. PARV4 was also identified in 14 out of 104 samples (13.5%) from lung transplant recipients, with no clear-cut association with available clinical markers. Point mutations located on the zone of real-time detection were identified for some amplification products. This study describes the detection of PARV4 in the blood of haemodialysis and lung transplanted patients with significant difference in prevalence in these two cohorts. Further studies will be needed in order to understand better both the potential implication in host health and the natural history of this virus.

Mots clés: PARV4; Parvoviridae; hemodialysis; lung transplant; epidemiology; genetic diversity

Domaines: Anthropologie biologique; Virologie

Mhammed Touinssi, Martine Reynaud-Gaubert, Carine Gomez, Pascal Thomas, Bertrand Dussol, et al.. Parvovirus 4 in French in-patients: a study of haemodialysis and lung transplant cohorts. Journal of Medical Virology, 2011, 83 (4), pp.717-721. ⟨10.1002/jmv.22003⟩. ⟨hal-00614692⟩