Combining various strategies to increase the coverage of the plant cell wall glycoproteome.

Abstract : Glycoproteomics recently became a very active field, mostly in mammals. The first part of this paper consists of a mini-review on the strategies used in glycoproteomics, namely methods for enrichment in glycoproteins and mass spectrometry (MS) techniques currently used. In a second part, these strategies are applied to the cell wall glycoproteome of etiolated hypocotyls of Arabidopsis thaliana, showing their complementarity. Several sub-glycoproteomes were obtained by: (i) affinity chromatography on concanavaline A (ConA) and analysis of glycoproteins by MALDI-TOF MS; (ii) multidimensional lectin chromatography (using AIL, PNA, ConA and WGA lectins) and subsequent identification of glycoproteins by MALDI-TOF MS and LC-MS/MS; (iii) boronic acid chromatography followed by identification of glycoproteins by MALDI-TOF MS. Altogether, 127 glycoproteins were identified. Most glycoproteins were found to be putative N-glycoproteins and N-glycopeptides were predicted from MS data using the ProTerNyc bioinformatics software.
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Journal articles
Phytochemistry, Elsevier, 2011, 72 (10), pp.1109-23. 〈10.1016/j.phytochem.2010.10.019〉
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Submitted on : Friday, July 22, 2011 - 3:29:59 PM
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Yu Zhang, Aurélie Giboulot, Michel Zivy, Benoît Valot, Elisabeth Jamet, et al.. Combining various strategies to increase the coverage of the plant cell wall glycoproteome.. Phytochemistry, Elsevier, 2011, 72 (10), pp.1109-23. 〈10.1016/j.phytochem.2010.10.019〉. 〈hal-00610579〉

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