Combining various strategies to increase the coverage of the plant cell wall glycoproteome.

Abstract : Glycoproteomics recently became a very active field, mostly in mammals. The first part of this paper consists of a mini-review on the strategies used in glycoproteomics, namely methods for enrichment in glycoproteins and mass spectrometry (MS) techniques currently used. In a second part, these strategies are applied to the cell wall glycoproteome of etiolated hypocotyls of Arabidopsis thaliana, showing their complementarity. Several sub-glycoproteomes were obtained by: (i) affinity chromatography on concanavaline A (ConA) and analysis of glycoproteins by MALDI-TOF MS; (ii) multidimensional lectin chromatography (using AIL, PNA, ConA and WGA lectins) and subsequent identification of glycoproteins by MALDI-TOF MS and LC-MS/MS; (iii) boronic acid chromatography followed by identification of glycoproteins by MALDI-TOF MS. Altogether, 127 glycoproteins were identified. Most glycoproteins were found to be putative N-glycoproteins and N-glycopeptides were predicted from MS data using the ProTerNyc bioinformatics software.
Document type :
Journal articles
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-00610579
Contributor : Elisabeth Jamet <>
Submitted on : Friday, July 22, 2011 - 3:29:59 PM
Last modification on : Monday, April 29, 2019 - 5:10:19 PM
Long-term archiving on : Friday, November 4, 2011 - 3:11:16 PM

Identifiers

Collections

Citation

Yu Zhang, Aurélie Giboulot, Michel Zivy, Benoît Valot, Elisabeth Jamet, et al.. Combining various strategies to increase the coverage of the plant cell wall glycoproteome.. Phytochemistry, Elsevier, 2011, 72 (10), pp.1109-23. ⟨10.1016/j.phytochem.2010.10.019⟩. ⟨hal-00610579⟩

Share

Metrics

Record views

660

Files downloads

1031