The Drosophila peptidoglycan-recognition protein LF interacts with peptidoglycan-recognition protein LC to downregulate the Imd pathway

Nada Basbous Franck Coste 1 Philippe Leone 2 Renaud Vincentelli 2 Julien Royet 3 Christine Kellenberger 1 Alain Roussel 1
1 CBM - Centre de biophysique moléculaire
2 AFMB - Architecture et fonction des macromolécules biologiques
3 RIDI - UPR 9002 - Réponse immunitaire et developpement chez les insectes
Abstract : The peptidoglycan (PGN)-recognition protein LF (PGRP-LF) is a specific negative regulator of the immune deficiency (Imd) pathway in Drosophila. We determine the crystal structure of the two PGRP domains constituting the ectodomain of PGRP-LF at 1.72 and 1.94 angstrom resolution. The structures show that the LFz and LFw domains do not have a PGN-docking groove that is found in other PGRP domains, and they cannot directly interact with PGN, as confirmed by biochemical-binding assays. By using surface plasmon resonance analysis, we show that the PGRP-LF ectodomain interacts with the PGRP-LCx ectodomain in the absence and presence of tracheal cytotoxin. Our results suggest a mechanism for downregulation of the Imd pathway on the basis of the competition between PRGP-LCa and PGRP-LF to bind to PGRP-LCx.
Keywords : IMMUNE-RESPONSE PGRP-LC BACTERIA RECEPTOR ACTIVATION Drosophila innate immunity structural biology
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Journal articles
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https://hal.archives-ouvertes.fr/hal-00608209
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Submitted on : Tuesday, July 12, 2011 - 2:29:13 PM
Last modification on : Wednesday, June 5, 2019 - 5:32:18 PM

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Nada Basbous, Franck Coste, Philippe Leone, Renaud Vincentelli, Julien Royet, et al.. The Drosophila peptidoglycan-recognition protein LF interacts with peptidoglycan-recognition protein LC to downregulate the Imd pathway. EMBO Reports, EMBO Press, 2011, 12 (4), pp.327-333. ⟨10.1038/embor.2011.19⟩. ⟨hal-00608209⟩

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