Asymmetrical stress generated by the erythrocyte lipid flippase triggers multiple bud formation on the surface of spherical giant liposomes.

Pierre Ezanno , Sophie Cribier (1, 2) , Philippe F Devaux

1 IBPC (FR_550) - Institut de biologie physico-chimique
2 UPMC - Université Pierre et Marie Curie - Paris 6

Proteo-giant liposomes were electroformed from a mixture of lecithin vesicles and inside-out vesicles from erythrocytes. After addition of Mg-ATP in the vicinity of the proteo-giant liposomes, small buds appeared on the liposome surfaces, which--via an increase in lipids in the outer monolayer--demonstrated the active transport of lipids from the inner to the outer monolayer, indicating flippase activity.

Domaines: Chimie organique

Pierre Ezanno, Sophie Cribier, Philippe F Devaux. Asymmetrical stress generated by the erythrocyte lipid flippase triggers multiple bud formation on the surface of spherical giant liposomes.. Biophysics of structure and mechanism, 2010, 39 ((8)), pp.1277-80. ⟨10.1007/s00249-009-0557-3⟩. ⟨hal-00594323⟩