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A role for the universal Kae1/Qri7/YgjD (COG0533) family in tRNA modification.

Abstract : The YgjD/Kae1 family (COG0533) has been on the top-10 list of universally conserved proteins of unknown function for over 5 years. It has been linked to DNA maintenance in bacteria and mitochondria and transcription regulation and telomere homeostasis in eukaryotes, but its actual function has never been found. Based on a comparative genomic and structural analysis, we predicted this family was involved in the biosynthesis of N(6)-threonylcarbamoyl adenosine, a universal modification found at position 37 of tRNAs decoding ANN codons. This was confirmed as a yeast mutant lacking Kae1 is devoid of t(6)A. t(6)A(-) strains were also used to reveal that t(6)A has a critical role in initiation codon restriction to AUG and in restricting frameshifting at tandem ANN codons. We also showed that YaeZ, a YgjD paralog, is required for YgjD function in vivo in bacteria. This work lays the foundation for understanding the pleiotropic role of this universal protein family.
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Contributor : Marie-Claude Serre <>
Submitted on : Monday, May 16, 2011 - 3:01:24 PM
Last modification on : Sunday, June 7, 2020 - 6:24:02 AM

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Basma El Yacoubi, Isabelle Hatin, Christopher Deutsch, Tamer Kahveci, Jean-Pierre Rousset, et al.. A role for the universal Kae1/Qri7/YgjD (COG0533) family in tRNA modification.. EMBO Journal, EMBO Press, 2011, 30 (5), pp.882-93. ⟨10.1038/emboj.2010.363⟩. ⟨hal-00593551⟩



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