Double-band sarcomeric SHG pattern induced by adult skeletal muscles alteration during myofibrils preparation. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Microscopy Année : 2011

Double-band sarcomeric SHG pattern induced by adult skeletal muscles alteration during myofibrils preparation.

Résumé

To understand the reported difference between double band, sarcomeric second harmonic generation pattern of isolated myofibril and predominant single band pattern found in thick muscle tissues, we studied the effect of myofibril preparation on the second harmonic generation pattern. We found that double band sarcomeric second harmonic generation pattern usually observed in myofibrils (prepared from fresh tissue) is due to muscle alteration during the mixing and triton treatment processes. Single band sarcomeric second harmonic generation pattern could be observed in isolated myofibrils when this alteration is previously prevented using paraformaldehyd fixed tissue. We conclude that single band sarcomeric second harmonic generation pattern is a signature of adult muscle myofibrils in normal physiological condition, suggesting that sarcomeric second harmonic generation patterns could be used as a valuable diagnosis tool of muscle health.
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Dates et versions

hal-00586716 , version 1 (18-04-2011)

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Gaëlle Recher, Denis Rouède, Christophe Tascon, Laure-Anne d'Amico, François Tiaho. Double-band sarcomeric SHG pattern induced by adult skeletal muscles alteration during myofibrils preparation.. Journal of Microscopy, 2011, 241 (2), pp.207-11. ⟨10.1111/j.1365-2818.2010.03425.x⟩. ⟨hal-00586716⟩
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