Love wave bacterial biosensors and microfluidic network for detection of heavy metal toxicity

Abstract : The presented work deals with the development of a bacteria-based Love wave sensor platform for the detection of heavy metals in liquid medium. The acoustic delay-line was inserted in an oscillation loop and associated with a Polydimethylsiloxane (PDMS) microfluidic network fabricated using soft photolithography and molding. It resulted in a small platform convenient for fast analysis and detection. Whole bacteria (Escherichia. coli) were fixed as bioreceptors onto the acoustic path of the sensor coated with a polyelectrolyte multilayer (PEM) using a simple and efficient layer by layer electrostatic self-assembly procedure. PEM and bacteria deposition were real-time characterized by monitoring the resonance frequency modification due to mass loading effect. Then when a solution of metal ion (Cd2+, Hg2+) was pumped through the microchannels, a significative frequency variation was observed down to 10-12 mol.l-1 concentration, due to changes of bacteria visco-elastic equivalent parameters in presence of toxic heavy metals.
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https://hal.archives-ouvertes.fr/hal-00584260
Contributor : Corinne Dejous <>
Submitted on : Friday, April 8, 2011 - 12:45:49 AM
Last modification on : Tuesday, February 27, 2018 - 11:18:10 PM

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Ibtissem Gammoudi, Hakim Tarbague, Jean-Luc Lachaud, Serge Destor, Ali Othmane, et al.. Love wave bacterial biosensors and microfluidic network for detection of heavy metal toxicity. Sensor letters, American Scientific Publishers, 2011, 9 (2), pp.816-818. ⟨10.1166/sl.2011.1621⟩. ⟨hal-00584260⟩

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