Comparative study of 3 screening tests, ExplorerÒ test, PremiÒTest2 microbiological tube tests and a multi-sulphonamides ELISA kit, for the detection of antimicrobial and sulphonamide residues in eggs

Valerie Gaudin (1) , Celine Hedou (2) , Annie Rault (2) , Pascal Sanders (2) , Eric Verdon (2)

1 LERMVD
2 LERMVD

The screening of antimicrobial residues in eggs is especially an important subject. Three different commercial kits for the screening of sulphonamides and other antimicrobials in eggs have been validated in accordance with the decision 2002/657/EC (EC 2002): one ELISA kit multi-sulphonamides (from RAISIO Diagnostics) and 2 microbiological tests (PremiÒTest from DSM and ExplorerÒ kit from Zeu-Inmunotec). The specificity was very satisfactory for all kits (false positive rates were lower than 2 % for all kits). The detection capabilities (CCb) have to be as low as possible for banned substances and lower than MRL when MRLs were set. The sensitivity of Premi®Test was better than sensitivity of Explorer® test, probably because of the eggs dilution before Explorer® test. The CCb values towards most of the tested sulphonamides were satisfactory with Premi®Test (£ 100 ng g-1µg kg-1). The participation to a proficiency test for the detection of sulphonamides in eggs with Premi®Test confirmed these results. The detection capability of tetracycline and doxycycline were at the level of MRL or 2 times the MRL maximum. The detection capabilities of chlortetracycline and oxytetracycline were higher (4 to 6 times the MRL). The detection capabilities for amoxicillin, neomycin, tylosin and erythromycin were lower than their respective MRLs. The participation to a proficiency test with Premi®Test gave very satisfactory results. Detection capabilities for sulphonamides were much lower for ELISA kit than for microbiological tests. The ELISA kit (from RAISIO Diagnostics) could be recommended for the targeted screening of sulphonamides in eggs. On the other hand, Explorer® test and Premi®Test could be used as wide screening tests which allowed to detect most of the antimicrobial families.

Mots clés: Life Sciences

Valerie Gaudin, Celine Hedou, Annie Rault, Pascal Sanders, Eric Verdon. Comparative study of 3 screening tests, ExplorerÒ test, PremiÒTest2 microbiological tube tests and a multi-sulphonamides ELISA kit, for the detection of antimicrobial and sulphonamide residues in eggs. Food Additives and Contaminants, 2009, 26 (04), pp.427-440. ⟨10.1080/02652030802527626⟩. ⟨hal-00577344⟩