AFNOR validation of PremiTest, a microbiological-based screening tube test for the detection of antimicrobial residues, in muscles from different animal origins
Résumé
Premi®Test contains viable spores of a strain of Bacillus stearothermophilus which is sensitive to antimicrobial residues such as beta-lactams, tetracyclines, macrolides, sulphonamides, ... The growth of the strain is inhibited by the presence of antimicrobial residues in muscle samples. PremiÒTest was validated according to AFNOR rules (French Association for Normalisation). The AFNOR validation was based on the comparison of a reference method (French Official method (Four Plate Test) and the STAR protocol (5 plate test)) with the alternative method (PremiÒTest). A preliminary study was conducted in an expert laboratory (Community Reference Laboratory CRL) on both spiked and incurred samples (field samples). Several criteria (sensitivity, specificity, relative accuracy) have been estimated and discussed, in addition to detection capabilities. Adequate agreement was found between alternative and reference method. However, PremiÒTest was more sensitive to beta-lactams and sulphonamides than the FPT. Later on, a collaborative study was organised by the CRL with 11 laboratories. Blank and spiked juicemeat juice samples were sent to the participants. The expert laboratory (CRL) statistically analysed the results. In conclusion, Premiâ Test could be used for the routine determination of antimicrobial residues in muscles from different animal origins with acceptable analytical performance. The detection capabilities of Premi®Test for beta-lactams (amoxicillin, ceftiofur), one macrolide (tylosin) and tetracycline were at the level of the respective Maximum Residue Limits (MRL) in muscle samples or even lower.
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