Inhibition of viral replication in vitro by antiviral-treated amniotic membrane.Possible use of amniotic membrane as drug-delivering tool.
Résumé
Purpose: To investigate if amniotic membrane (AM) incubated with antivirals can inhibit viral growth in vitro. Methods: AM samples were incubated with a solution of acyclovir (ACV) or trifluridine (TFU). AM treated was placed onto a monolayers of Vero cells, a continuous cell line from monkey kidney, infected with Herpes Simplex Virus (HSV). Viral growth was assessed in comparison to control infected cells by direct examination with an inverted microscope at low magnification for the presence and extension of the typical cytopathic effect, or by estimation of viral genomes. Results: AM soaked in ACV or TFU inhibited significantly the development of HSV in cell cultures, based on the viral growth compared to controls. Non-treated AM did not significantly affect viral replication. Conclusions: Our preliminary in vitro data show that antiviral-treated amniotic membrane can inhibit viral replication. Therefore, it could be taken into consideration the possibility to combine the previously published anti-inflammatory properties of AM with the capability to absorb antivirals and sustain drug release.
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