Redistribution of meta-tetra(hydroxyphenyl)chlorin (m-THPC) from conventional and PEGylated liposomes to biological substrates

Abstract : We used the phenomenon of previously described photoinduced fluorescence quenching and fluorescence polarization to evaluate the transfer of meta-tetra(hydroxyphenyl)chlorin (m-THPC) from commercial high-drug load liposomes to plasma proteins and model membranes. Fluorescence quenching of m-THPC in liposomes by iodide indicates that part of m-THPC in PEGylated liposomes is localized in the PEG shell, while the rest is bound to the lipid bilayer. It was shown that the two molecule pools in the commercial PEGylated liposomal formulation Fospeg® condition the characteristics of the m-THPC release kinetics. A substantial percentage of m-THPC from Fospeg® is released much faster than from the conventional liposomal formulation Foslip®. Using the technique of resonance light scattering, it was shown that partial m-THPC aggregation is present in liposomes with very high drug loads, higher in PEGylated liposomes compared to conventional ones.
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Photochemical & Photobiological Sciences Photochemical and Photobiological Sciences, Royal Society of Chemistry, 2011, 10 (6), pp.911-919. 〈10.1039/C0PP00303D〉
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https://hal.archives-ouvertes.fr/hal-00574572
Contributeur : Marie Ange D'Hallewin <>
Soumis le : mardi 8 mars 2011 - 13:35:17
Dernière modification le : jeudi 19 mai 2016 - 01:14:49

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Vadim Reshetov, Dzmitry Kachatkou, Tatiana Shmigol, Vladimir Zorin, Marie Ange D'Hallewin, et al.. Redistribution of meta-tetra(hydroxyphenyl)chlorin (m-THPC) from conventional and PEGylated liposomes to biological substrates. Photochemical & Photobiological Sciences Photochemical and Photobiological Sciences, Royal Society of Chemistry, 2011, 10 (6), pp.911-919. 〈10.1039/C0PP00303D〉. 〈hal-00574572〉

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