Fluorescence methods for detecting the kinetics of photosensitizer release from nanosized carriers

Vadim Reshetov (1, 2, 3) , Tatyana Zorina (3) , Marie Ange d'Hallewin (1, 2) , Lina Bezdetnaya (1, 2) , Vladimir Zorin (3)

1 CRAN - Centre de Recherche en Automatique de Nancy
2 CAV - Centre Alexis Vautrin
3 Laboratory of Biophysics and Biotechnology [Belarusian]

Three approaches to analyzing the rate of release of the photosensitizer meta-tetrahydroxyphenylchlorin (mTHPC) from unilaminar lipid vesicles (ULV) in model biological systems are studied: by excitation energy transfer from probe diphenylhexatriene to mTHPC, by the fluorescence anisotropy of mTHPC, and by photoinduced quenching of the fluorescence of mTHPC. Each of these methods has its characteristic range of sensitivity for measurements of the local concentration of mTHPC in ULV. Fluorescence anisotropy can be used for quantitative determination of the mTHPC yield from ULV for mTHPC:lipid ratios of 1:100 to 1:1000, determining the efficiency of fluorescence quenching of diphenylhexatriene for ratios <1:200, and photoinduced quenching for ratios of 1:10-1:500.

Domaines: Biophysique

Vadim Reshetov, Tatyana Zorina, Marie Ange d'Hallewin, Lina Bezdetnaya, Vladimir Zorin. Fluorescence methods for detecting the kinetics of photosensitizer release from nanosized carriers. Journal of Applied Spectroscopy, 2011, 78 (1), pp.103-109. ⟨10.1007/s10812-011-9431-z⟩. ⟨hal-00574570⟩