Love wave biosensor for real-time detection of okadaic acid as DSP phycotoxin

Abstract : This paper reports the detection of okadaic acid (OA) as a Diarrheic Shellfish Poisoning (DSP) toxin with an acoustic wave platform in real-time. According to the FDA, the threshold for safe consumption of shellfish is 20 μg of OA for 100 g of shellfish tissue. The high gravimetric sensitivity of Love wave acoustic devices allow, in liquid media, immuno-detection thanks to immobilized specific antibodies. The biosensor is composed of two lines, one for the test and one used as a reference. Sensitive films were deposited through a PolyDiMethylSiloxane (PDMS) microfluidic chip. On both lines, anti-okadaic acid antibodies (OA-Ab) and saturating agents were successively injected under continuous flow with controlled flow rate. On the test line, okadaic acid (OA) was injected while an unspecific peptide (6×Histidine, 6×His) was used on the control line. On both lines, polyclonal OA-Ab were re-injected a second time to reveal previously fixed OA on the test line. Measured frequency shifts were three times higher on test lines than on control lines. In these conditions, for only 2 μg of OA used for detection, the acoustic wave platform could detect DSP toxins with only 10 g of shellfish tissue.
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https://hal.archives-ouvertes.fr/hal-00570368
Contributor : Corinne Dejous <>
Submitted on : Monday, February 28, 2011 - 1:19:44 PM
Last modification on : Tuesday, February 27, 2018 - 11:18:01 PM

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Fabien Fournel, Etienne Baco, Maria Mamani-Matsuda, Marie Degueil, Bernard Bennetau, et al.. Love wave biosensor for real-time detection of okadaic acid as DSP phycotoxin. Sensors and Actuators B: Chemical, Elsevier, 2012, 170, pp.122-128. ⟨10.1016/j.snb.2011.02.056⟩. ⟨hal-00570368⟩

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