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A potentially immunologically inert derivative of the reverse tetracycline-controlled transactivator

Abstract : The archetypical system for regulating heterologous gene expression in mammalian cells involves tetracycline-activated transactivators (rtTA). Binding of such transactivators to tet-operator-controlled promoters induces transcription. Immune responses directed against the transactivator proteins may limit the applicability of this system in immune-competent hosts. To circumvent such immune responses the immune evasion mechanism of the Epstein–Barr virus Nuclear-Antigen 1 was exploited. Our data show that fusion of the rtTA with the EBNA-1 derived Gly-Ala repeat yielded an efficient transactivator with no detectable activity in absence of inducer. Antigenic peptides of the fusion protein were not presented in MHC class I.
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Submitted on : Sunday, February 13, 2011 - 2:50:54 AM
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Arnaud Zaldumbide, Susan Weening, Steve J. Cramer, Martijn J. W. E. Rabelink, Joost Verhaagen, et al.. A potentially immunologically inert derivative of the reverse tetracycline-controlled transactivator. Biotechnology Letters, Springer Verlag, 2010, 32 (6), pp.749-754. ⟨10.1007/s10529-010-0218-8⟩. ⟨hal-00565435⟩

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