Flow cytometry and spectral imaging multiphoton microscopy analysis of CD36 expression with quantum dots 605 of untreated and 7-ketocholesterol-treated human monocytic cells.

Edmond Kahn (1) , Franck Ménétrier , Anne Vejux (2, 3) , Thomas Montange , Dominique Dumas (4, 5) , Jean-Marc Riedinger (6) , Frédérique Frouin (1) , Yves Tourneur (7) , Frédéric Brau (8) , Jean-François Stoltz (4) , Gérard Lizard (3)

1 LIF - Laboratoire d'Imagerie Fonctionnelle
2 LBMC - Laboratoire de Biochimie Moléculaire et Cellulaire
3 LNC - Lipides - Nutrition - Cancer (U866)
4 LEMTA - Laboratoire d'Energétique et Mécanique Théorique et Appliquée
5 PTIBC-IBISA Nancy - Plate-forme Imagerie et Biophysique Cellulaire et Tissulaire
6 Laboratoire de Biologie Médicale [Centre Georges-François leclerc]
7 Centre Commun de Quantimétrie
8 IPMC - Institut de pharmacologie moléculaire et cellulaire

QDs 605 are useful tools to perform antigenic expression in FCM and CLSM. Moreover, CLSM and subsequent spectral analysis provide a more specific characterization of QDs 605 fluorescent emission in the UV excitation mode and a simultaneous identification of 7KC.

Domaines: Biochimie, Biologie Moléculaire

Edmond Kahn, Franck Ménétrier, Anne Vejux, Thomas Montange, Dominique Dumas, et al.. Flow cytometry and spectral imaging multiphoton microscopy analysis of CD36 expression with quantum dots 605 of untreated and 7-ketocholesterol-treated human monocytic cells.. Analytical and Quantitative Cytology and Histology, 2006, 28 (6), pp.316-30. ⟨hal-00559053⟩