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Article Dans Une Revue Cell Année : 2010

Single-stranded DNA transposition is coupled to host replication.

Résumé

DNA transposition has contributed significantly to evolution of eukaryotes and prokaryotes. Insertion sequences (ISs) are the simplest prokaryotic trans-posons and are divided into families on the basis of their organization and transposition mechanism. Here, we describe a link between transposition of IS608 and ISDra2, both members of the IS200/IS605 family, which uses obligatory single-stranded DNA intermediates, and the host replication fork. Replication direction through the IS plays a crucial role in excision: activity is maximal when the "top'' IS strand is located on the lagging-strand template. Excision is stimulated upon transient inactivation of replicative helicase function or inhibition of Okazaki fragment synthesis. IS608 insertions also exhibit an orientation preference for the lagging-strand template and insertion can be specifically directed to stalled replication forks. An in silico genomic approach provides evidence that dissemination of other IS200/IS605 family members is also linked to host replication.

Dates et versions

hal-00557930 , version 1 (20-01-2011)

Identifiants

Citer

B. Ton-Hoang, C. Pasternak, P. Siguier, Catherine Guynet, A.B. Hickman, et al.. Single-stranded DNA transposition is coupled to host replication.. Cell, 2010, 142 (3), pp.398-408. ⟨10.1016/j.cell.2010.06.034⟩. ⟨hal-00557930⟩
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