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Journal articles
Regulation of splicing enhancer activities by RNA secondary structures
Wei Liu
1
Yu Zhou
1, 2, 3
Zexi Hu
1
Tao Sun
1
Alain Denise
2, 4, 5
Xiang-Dong Fu
1, 3
Yi Zhang
1, 6
5
AMIB - Algorithms and Models for Integrative Biology
LIX - Laboratoire d'informatique de l'École polytechnique [Palaiseau], LRI - Laboratoire de Recherche en Informatique, UP11 - Université Paris-Sud - Paris 11, Inria Saclay - Ile de France
Alain Denise 2, 4, 5
Mail :
Author IdHAL : alain-denise
Xiang-Dong Fu 1, 3
Author
Yi Zhang 1, 6
Author
1
State Key Laboratory of Virology (State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei 430072, China - China)
- Wuhan University [China] (Wuchang, Wuhan, Hubei, China, 430072 - China)
2
LRI - Laboratoire de Recherche en Informatique (LRI - Bâtiments 650-660 Université Paris-Sud 91405 Orsay Cedex - France)
- CNRS - Centre National de la Recherche Scientifique : UMR8623 (France)
- UP11 - Université Paris-Sud - Paris 11 (Bâtiment 300 - 91405 Orsay cedex - France)
- CentraleSupélec (3, rue Joliot Curie, Plateau de Moulon, 91192 GIF-SUR-YVETTE Cedex - France)
3
CMM - Department of Cellular and Molecular Medicine (University of California, San Diego Dept. of Cellular and Molecular Medicine 9500 Gilman Dr. MC 0651 La Jolla, CA 92093-0651 - United States)
- UC San Diego - University of California [San Diego] (9500 Gilman Dr., La Jolla, CA 92093 USA - United States)
- University of California (United States)
4
IGM - Institut de génétique et microbiologie [Orsay] (Bâtiments 400 et 409
UFR des Sciences - Université Paris-Sud 11
15, rue Georges Clémenceau - F 91405 ORSAY Cedex - France)
- CNRS - Centre National de la Recherche Scientifique : UMR8621 (France)
- UP11 - Université Paris-Sud - Paris 11 (Bâtiment 300 - 91405 Orsay cedex - France)
5
AMIB - Algorithms and Models for Integrative Biology (Algorithmes et modèles pour la Biologie Intégrative
Bâtiment Alan Turing - Campus de l'École Polytechnique - 1 rue Honoré d'Estienne d'Orves - 91120 Palaiseau - France)
- LIX - Laboratoire d'informatique de l'École polytechnique [Palaiseau] (Route de Saclay 91128 PALAISEAU CEDEX - France)
- CNRS - Centre National de la Recherche Scientifique : UMR7161 (France)
- X - École polytechnique (École polytechnique, 91128 Palaiseau Cedex - France)
- LRI - Laboratoire de Recherche en Informatique (LRI - Bâtiments 650-660 Université Paris-Sud 91405 Orsay Cedex - France)
- CNRS - Centre National de la Recherche Scientifique : UMR8623 (France)
- UP11 - Université Paris-Sud - Paris 11 (Bâtiment 300 - 91405 Orsay cedex - France)
- CentraleSupélec (3, rue Joliot Curie, Plateau de Moulon, 91192 GIF-SUR-YVETTE Cedex - France)
- UP11 - Université Paris-Sud - Paris 11 (Bâtiment 300 - 91405 Orsay cedex - France)
- Inria Saclay - Ile de France (1 rue Honoré d'Estienne d'Orves
Bâtiment Alan Turing
Campus de l'École Polytechnique
91120 Palaiseau - France)
- Inria - Institut National de Recherche en Informatique et en Automatique (Domaine de Voluceau Rocquencourt - BP 105 78153 Le Chesnay Cedex - France)
6
ABLife Inc (Building E-1301, 115 Guangba Road, Wuhan, Hubei 430072, China - China)
- ABLife Inc (France)
Abstract : In this report, we studied the effect of RNA structures on the activity of exonic splicing enhancers on the SMN1 minigene model by engineering known ESEs into different positions of stable hairpins. We found that as short as 7-bp stem is sufficient to abolish the enhancer activity. When placing ESEs in the loop region, AG-rich ESEs are fully active, but a UCG-rich ESE is not because of additional structural constraints. ESEs placed adjacent to the 3' end of the hairpin structure display high enhancer activity, regardless of their sequence identities. These rules explain the suppression of multiple ESEs by point mutations that result in a stable RNA structure, and provide an additional mechanism for the C6T mutation in SMN2.
Complete list of metadatas
https://hal.archives-ouvertes.fr/hal-00534366
Contributor : Marie-Claude Serre <>
Submitted on : Tuesday, November 9, 2010 - 2:15:00 PM
Last modification on : Tuesday, April 21, 2020 - 1:11:04 AM
Contributor : Marie-Claude Serre <>
Submitted on : Tuesday, November 9, 2010 - 2:15:00 PM
Last modification on : Tuesday, April 21, 2020 - 1:11:04 AM
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Identifiers
- HAL Id : hal-00534366, version 1
- DOI : 10.1016/j.febslet.2010.09.039
- PUBMED : 20888818
Citation
Wei Liu, Yu Zhou, Zexi Hu, Tao Sun, Alain Denise, et al.. Regulation of splicing enhancer activities by RNA secondary structures. FEBS Letters, Wiley, 2010, 584 (21), pp.4401-7. ⟨10.1016/j.febslet.2010.09.039⟩. ⟨hal-00534366⟩
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