Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2010

Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution.

Otto Berninghausen
Birgitta Beatrix
Eric Westhof
Roland Beckmann

Résumé

Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant (Triticum aestivum) 80S ribosome at 5.5-Å resolution. This map, together with a 6.1-Å map of a Saccharomyces cerevisiae 80S ribosome, has enabled us to model ∼98% of the rRNA. Accurate assignment of the rRNA expansion segments (ES) and variable regions has revealed unique ES-ES and r-protein-ES interactions, providing insight into the structure and evolution of the eukaryotic ribosome.

Dates et versions

hal-00530586 , version 1 (29-10-2010)

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Jean-Paul Armache, Alexander Jarasch, Andreas M Anger, Elizabeth Villa, Thomas S. Becker, et al.. Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution.. Proceedings of the National Academy of Sciences of the United States of America, 2010, epub ahead of print. ⟨10.1073/pnas.1009999107⟩. ⟨hal-00530586⟩
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