Tools for stools: the challenge of assessing human intestinal microbiota using molecular diagnostics - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Expert Review of Molecular Diagnostics Année : 2009

Tools for stools: the challenge of assessing human intestinal microbiota using molecular diagnostics

Résumé

The human GI tract is inhabited by an incredibly complex and abundant microbiota, whose composition is dependent on a variety of factors. The gut microbiota has an influence in the morphological, immunological and nutritional functions of the digestive tract and may be involved in many diseases. This article proposes the rationale behind conducting in vitro diagnostics (IVDs) of the human microbiota, as well as outlining the conceptual and technical difficulties involved in IVD testing. The molecular methods that can be used according to whether the IVD tools are employed to study one individual constituent species or to determine the microbiota as a whole will also be described. In the latter case, these technologies include high-throughput sequencing for metagenomics and DNA microarrays, which can now be efficiently used to study gut ecology and are believed to represent the future of standardized diagnostics.
Fichier non déposé

Dates et versions

hal-00529610 , version 1 (26-10-2010)

Identifiants

Citer

Jean-François Brugère, Agnès Mihajlovski, Mohieddine Missaoui, Pierre Peyret. Tools for stools: the challenge of assessing human intestinal microbiota using molecular diagnostics. Expert Review of Molecular Diagnostics, 2009, 9 (4), pp.353-65. ⟨10.1586/erm.09.16⟩. ⟨hal-00529610⟩
121 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More