Ultrasound-assisted microbubbles gene transfer in tendons for gene therapy

Abstract : Our study aimed at evaluating the use of ultrasound-assisted microbubbles gene transfer in mice Achilles tendons. Using a plasmid encoding luciferase gene, it was found that an efficient and stable gene expression for more than two weeks was obtained when tendons were injected with 10 mu g of plasmid in the presence of 5 x 10(5) BR14 microbubbles with the following acoustic parameters: 1 MHz, 200 kPa, 40% duty cycle and 10 min of exposure time. The rate of gene expression was 100-fold higher than that obtained with naked plasmid injected alone without ultrasound or with ultrasound in absence of microbubbles. The long term expression of transgene makes ultrasound-assisted microbubble a suitable method for gene therapy in tendons.
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Submitted on : Monday, October 25, 2010 - 2:04:01 PM
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Anthony Delalande, Michel-Francis Bureau, Patrick Midoux, Ayache Bouakaz, Chantal Pichon. Ultrasound-assisted microbubbles gene transfer in tendons for gene therapy. Ultrasonics Sonochemistry, Elsevier, 2010, 50 (2), pp.269-272. ⟨10.1016/j.ultras.2009.09.035⟩. ⟨hal-00529321⟩



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