Lipid Nanotube Encapsulating Method in Low-Energy Scanning Transmission Electron Microscopy Analyses

Abstract : The lipid nanotube (LNT) encapsulating method is a rational sample fixation method that can be used to mount samples for transmission electron microscopy analyses. By employing the LNT encapsulating method in 30 kV low-voltage scanning transmission electron microscopy (LV-STEM), it is possible to record multiangle images of ferritin without using the negative staining method. We have also recorded a tilted series of high-contrast LV-STEM images and reconstructed three-dimensional images. These results show that LNTs have sufficient durability for LV electron beam, and indicate the potential of the LNT encapsulating method as a sample fixation method of LV electron microscopy.
keyword : MOLECULES
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Journal articles
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https://hal.archives-ouvertes.fr/hal-00522415
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Submitted on : Thursday, September 30, 2010 - 4:00:05 PM
Last modification on : Wednesday, June 27, 2018 - 8:24:02 AM

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Hirotoshi Furusho, Yumiko Mishima, Naohiro Kameta, Midori Yamane, Mitsutoshi Masuda, et al.. Lipid Nanotube Encapsulating Method in Low-Energy Scanning Transmission Electron Microscopy Analyses. Japanese Journal of Applied Physics, part 1 : Regular papers, Short Notes, 2009, 48 (9 part 1), pp.097001. ⟨10.1143/JJAP.48.097001⟩. ⟨hal-00522415⟩

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