Structure and expression analysis of rice paleo duplications

Mickael Throude (1) , Stéphanie Bolot (1) , Mickael Bosio (2) , Caroline Pont (1) , Xavier Sarda (2) , Umar Masood Quraishi (1) , Fabienne Bourgis (3) , Philippe Lessard (2) , Peter Rogowsky (4) , Alain Ghesquiere (3) , Alain Murigneux (2) , Gilles Charmet (1) , Pascual Perez (2) , Jerome Salse (1)

1 GDEC - Génétique Diversité et Ecophysiologie des Céréales
2 BIOGEMMA
3 IRD - Institut de Recherche pour le Développement
4 RDP - Reproduction et développement des plantes

Having a well-known history of genome duplication, rice is a good model for studying structural and functional evolution of paleo duplications. Improved sequence alignment criteria were used to characterize 10 major chromosome-to-chromosome duplication relationships associated with 1440 paralogous pairs, covering 47.8 of the rice genome, with 12.6 of genes that are conserved within sister blocks. Using a micro-array experiment, a genome-wide expression map has been produced, in which 2382 genes show significant differences of expression in root, leaf and grain. By integrating both structural (1440 paralogous pairs) and functional information (2382 differentially expressed genes), we identified 115 paralogous gene pairs for which at least one copy is differentially expressed in one of the three tissues. A vast majority of the 115 paralogous gene pairs have been neofunctionalized or subfunctionalized as 88, 89 and 96 of duplicates, respectively, expressed in grain, leaf and root show distinct expression patterns. On the basis of a Gene Ontology analysis, we have identified and characterized the gene families that have been structurally and functionally preferentially retained in the duplication showing that the vast majority (85) of duplicated have been either lost or have been subfunctionalized or neofunctionalized during 5070 million years of evolution.

Mots clés: RIZ; GENETIQUE

Domaines: Biochimie [q-bio.BM]

Mickael Throude, Stéphanie Bolot, Mickael Bosio, Caroline Pont, Xavier Sarda, et al.. Structure and expression analysis of rice paleo duplications. Nucleic Acids Research, 2009, 37 (4), pp.1248-1259. ⟨10.1093/nar/gkn1048⟩. ⟨hal-01189459⟩