Calmodulin-induced conformational and hydrodynamic changes in the catalytic domain of Bordetella pertussis adenylate cyclase toxin.

Johanna C Karst (1) , Ana Cristina Sotomayor Pérez (1) , J Iñaki Guijarro (2) , Bertrand Raynal (3) , Alexandre Chenal (1) , Daniel Ladant (1)

1 Biochimie des Interactions Macromoléculaires / Biochemistry of Macromolecular Interactions
2 Résonance Magnétique Nucléaire des Biomolécules
3 Biophysique des Macromolécules et de leurs Interactions

Bordetella pertussis, the causative agent of whooping cough, secretes among various toxins an adenylate cyclase (CyaA) that displays a unique mechanism of cell invasion, which involves a direct translocation of its N-terminal catalytic domain (AC, 400 residues) across the plasma membrane of the eukaryotic targeted cells. Once into the cytosol, AC is activated by endogenous calmodulin and produces toxic amounts of cAMP. The structure of AC in complex with the C-terminal part of calmodulin has recently been determined. However, as the structure of the catalytic domain in the absence of calmodulin is still lacking, the molecular basis of AC activation by calmodulin remains largely unknown. To characterize this activation mechanism, we investigated here the biophysical properties of the isolated catalytic domain in solution with or without calmodulin. We found that calmodulin triggered only minor modifications of the protein secondary and tertiary structure but had a pronounced effect on the hydrodynamic properties of AC. Indeed, while the isolated catalytic domain was spherical and hydrated, it underwent a significant elongation as well as compaction and dehydration upon calmodulin interaction. On the basis of these data, we propose a model for the structural transition between the calmodulin-free and calmodulin-bound AC.

Domaines: Biochimie, Biologie Moléculaire

Johanna C Karst, Ana Cristina Sotomayor Pérez, J Iñaki Guijarro, Bertrand Raynal, Alexandre Chenal, et al.. Calmodulin-induced conformational and hydrodynamic changes in the catalytic domain of Bordetella pertussis adenylate cyclase toxin.. Biochemistry, 2010, 49 (2), pp.318-28. ⟨10.1021/bi9016389⟩. ⟨hal-00512114⟩