Metabolic capacities in cultured human hepatocytes obtained by a new isolating procedure from non-wedge small liver biopsies.

Abstract : A new isolating procedure of human hepatocytes has been developed using two-step collagenase digestion by a non-perfusion procedure (NP) of non-wedge liver biopsies. 1. A yield of 2-7 x 10(6) hepatocytes/g liver, 52-95% viability and 13-75% attachment were obtained from liver biopsies weighing 6-60 g, comparable to that obtained when using the classical perfusion procedure (P) to isolate human hepatocytes from wedge liver samples of 50-150 g. 2. In culture, human hepatocytes obtained by NP remained attached to plastic for up to 5 days and displayed the usual morphological characteristics. Their metabolic capacities, assessed by liver-specific albumin and urea synthesis and by CYP-dependent and conjugation pathways, were equivalent to those of human hepatocytes obtained by P. In addition, they responded adequately to specific CYP inducers, demonstrating that they constitute a model in which human drug metabolism and toxicity studies can be performed.
Document type :
Journal articles
Human & Experimental Toxicology / Human and Experimental Toxicology, 1998, 17 (10), pp.544-553


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Submitted on : Thursday, July 15, 2010 - 4:25:23 PM
Last modification on : Thursday, July 15, 2010 - 4:25:23 PM

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  • HAL Id : hal-00502782, version 1
  • PUBMED : 9821017

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P. David, C. Viollon, E. Alexandre, A. Azimzadeh, L. Nicod, et al.. Metabolic capacities in cultured human hepatocytes obtained by a new isolating procedure from non-wedge small liver biopsies.. Human & Experimental Toxicology / Human and Experimental Toxicology, 1998, 17 (10), pp.544-553. <hal-00502782>

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