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Galectin-3, a marker for vacuole lysis by invasive pathogens.

Abstract : Shigella bacteria invade macrophages and epithelial cells and following internalization lyse the phagosome and escape to the cytoplasm. Galectin-3, an abundant protein in macrophages and epithelial cells, belongs to a family of beta-galactoside-binding proteins, the galectins, with many proposed functions in immune response, development, differentiation, cancer and infection. Galectins are synthesized as cytosolic proteins and following non-classical secretion bind extracellular beta-galactosides. Here we analysed the localization of galectin-3 following entry of Shigella into the cytosol and detected a striking phenomenon. Very shortly after bacterial invasion, intracellular galectin-3 accumulated in structures in vicinity to internalized bacteria. By using immuno-electron microscopy analysis we identified galectin-3 in membranes localized in the phagosome and in tubules and vesicles that derive from the endocytic pathway. We also demonstrated that the binding of galectin-3 to host N-acetyllactosamine-containing glycans, was required for forming the structures. Accumulation of the structures was a type three secretion system-dependent process. More specifically, existence of structures was strictly dependent upon lysis of the phagocytic vacuole and could be shown also by Gram-positive Listeria and Salmonella sifA mutant. We suggest that galectin-3-containing structures may serve as a potential novel tool to spot vacuole lysis.
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https://hal.archives-ouvertes.fr/hal-00486248
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Submitted on : Tuesday, May 25, 2010 - 2:04:00 PM
Last modification on : Tuesday, September 13, 2022 - 12:46:07 PM

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Irit Paz, Martin Sachse, Nicolas Dupont, Joelle Mounier, Cecilia Cederfur, et al.. Galectin-3, a marker for vacuole lysis by invasive pathogens.. Cellular Microbiology, Wiley, 2010, 12 (4), pp.530-44. ⟨10.1111/j.1462-5822.2009.01415.x⟩. ⟨hal-00486248⟩

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