Cell model of inflammation

Abstract : Chemical and physical stimuli trigger a cutaneous response by first inducing the main epidermal cells: keratinocytes, to produce specific mediators that are responsible for the initiation of skin inflammation. Activation modulates cell communications namely leukocyte recruitment and blood to skin extravasation through vascular endothelial cells selective barrier. We describe here an in vitro model which takes into account those various steps of human skin inflammation from keratinocyte activation up to the adhesion of leukocytes to dermal capillaries endothelial cells (ECs). Human adult keratinocytes (HAK) were stressed by exposure to ultraviolet-irradiation (UV), or neuropeptides then, the conditioned culture medium was used to mimic the natural micro-environmental conditions for dermal ECs. A relevant skin in vitro model must include appropriate cells from the skin. This is shown here by the selective reactions of dermal ECs as compared to EC lines from distinct origins in terms of leukocytes recruitment, sensitivity to stress, nature of the stress-induced secreted mediators. This simplified model is suitable to the screening of anti-inflammatory molecules whose activity involves the partnership of various skin cells.
keyword : Life Sciences
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Jocelyne Franchi, Clarisse Marteau, Claire Crola da Silva, Michèle Mitterrand, Patrice André, et al.. Cell model of inflammation. Bioscience Reports, Portland Press, 2008, 28 (1), pp.23-32. ⟨10.1042/BSR20070012⟩. ⟨hal-00479292⟩

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