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Article Dans Une Revue Biochemical Journal Année : 2007

Cargo-dependent cytotoxicity and delivery efficacy of cell-penetrating peptides: a comparative study

Samir El-Andaloussi
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Peter Järver
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Henrik J Johansson
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Ülo Langel
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Résumé

The use of cell-penetrating peptides (CPPs) as delivery vectors for bioactive molecules has been an emerging field since 1994 when the first CPP, penetratin, was discovered. Since then, several CPPs including the widely used Tat peptide, have been developed and utilized to translocate a wide range of compounds across the plasma membrane of cells both in vivo and in vitro. Although the field has emerged as a possible future candidate for drug delivery, little attention has been given to the potential toxic side-effects these peptides might exhibit in cargo delivery. Also, no comprehensive study has been performed to evaluate the relative efficacy of single CPPs to convey different cargos. Therefore, we selected three of the major CPPs; penetratin, Tat, and transportan 10, and evaluated their ability to deliver commonly used cargos, including fluoresceinyl moiety, double stranded DNA, and proteins (ie. avidin and streptavidin), and studied their effect on membrane integrity and cell viability. Our results demonstrate the unfeasibility to use translocation efficacy of fluorescein moiety as a gauge for CPP efficiency since the delivery properties are depending on cargo used. Further, and not less important, toxicity caused by CPPs depend heavily on peptide concentration, cargo molecule, and coupling strategy.

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Dates et versions

hal-00478786 , version 1 (30-04-2010)

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Samir El-Andaloussi, Peter Järver, Henrik J Johansson, Ülo Langel. Cargo-dependent cytotoxicity and delivery efficacy of cell-penetrating peptides: a comparative study. Biochemical Journal, 2007, 407 (2), pp.285-292. ⟨10.1042/BJ20070507⟩. ⟨hal-00478786⟩

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