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The cspA mRNA is a thermosensor that modulates translation of the cold-shock protein CspA.

Abstract : Cold induction of cspA, the paradigm Escherichia coli cold-shock gene, is mainly subject to posttranscriptional control, partly promoted by cis-acting elements of its transcript, whose secondary structure at 37 degrees C and at cold-shock temperature has been elucidated here by enzymatic and chemical probing. The structures, which were also validated by mutagenesis, demonstrate that cspA mRNA undergoes a temperature-dependent structural rearrangement, likely resulting from stabilization in the cold of an otherwise thermodynamically unstable folding intermediate. At low temperature, the "cold-shock" structure is more efficiently translated and somewhat less susceptible to degradation than the 37 degrees C structure. Overall, our data shed light on a molecular mechanism at the basis of the cold-shock response, indicating that cspA mRNA is able to sense temperature downshifts, adopting functionally distinct structures at different temperatures, even without the aid of trans-acting factors. Unlike with other previously studied RNA thermometers, these structural rearrangements do not result from melting of hairpin structures.
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https://hal.archives-ouvertes.fr/hal-00475727
Contributor : Isabelle Martin <>
Submitted on : Thursday, April 22, 2010 - 5:08:01 PM
Last modification on : Friday, November 6, 2020 - 5:30:04 PM

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Anna Maria Giuliodori, Fabio Di Pietro, Stefano Marzi, Benoit Masquida, Rolf Wagner, et al.. The cspA mRNA is a thermosensor that modulates translation of the cold-shock protein CspA.. Molecular Cell, Elsevier, 2010, 37 (1), pp.21-33. ⟨10.1016/j.molcel.2009.11.033⟩. ⟨hal-00475727⟩

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