Three distinct sarcomeric patterns of skeletal muscle revealed by SHG and TPEF microscopy.

Gaëlle Recher (1) , Denis Rouède (2) , Patrick Richard (2) , Antoine Simon (3) , Jean-Jacques Bellanger (3) , François Tiaho (1)

1 ICM - Interactions cellulaires et moléculaires
2 IPR - Institut de Physique de Rennes
3 LTSI - Laboratoire Traitement du Signal et de l'Image

We have extensively characterized the sarcomeric SHG signal as a function of animal species (rat versus xenopus), age (adult versus larval) and tissue preparation (fixed or fresh) and we found that the main feature of this signal is a single peak per mature sarcomere (about 85% of all sarcomeres). The remaining (15%) was found to be either double peak per mature sarcomere or mini sarcomeres (half of a sarcomere) using alpha-actinin immuno detection of the Z-band. The mini sarcomeres are often found in region of pitchfork-like SHG pattern. We suggest that double peak SHG pattern could indicate regions of sarcomeric proteolysis whereas pitchfork-like SHG pattern could reveal sarcomeric assembly.

Domaines: Imagerie; Optique / photonique

Gaëlle Recher, Denis Rouède, Patrick Richard, Antoine Simon, Jean-Jacques Bellanger, et al.. Three distinct sarcomeric patterns of skeletal muscle revealed by SHG and TPEF microscopy.. Optics Express, 2009, 17 (22), pp.19763-77. ⟨10.1364/OE.17.019763⟩. ⟨hal-00444944⟩