A versatile method for direct and covalent immobilization of DNA and proteins on biochips

B.P. Corgier , A. Laurent , Pascal Perriat (1) , Loiec Blum , Christophe A. Marquette

1 MATEIS - Matériaux, ingénierie et science [Villeurbanne]

(Figure Presented) Stuck on sensing: A method is presented for the immobilization of aryl diazonium modified biomolecules by direct electro-grafting onto the surface of a conducting material to create effective on-chip sensing layers (see picture). Proof of concept is given for both proteins (antibodies, enzymes) and DNA

Domaines: Matériaux

B.P. Corgier, A. Laurent, Pascal Perriat, Loiec Blum, Christophe A. Marquette. A versatile method for direct and covalent immobilization of DNA and proteins on biochips. Angewandte Chemie International Edition, 2007, pp.4108-4110. ⟨hal-00434118⟩